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KMID : 0903519660070010029
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1966 Volume.7 No. 1 p.29 ~ p.33
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Estimating urinary energy value of rat from the urinary nitrogen content
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Abstract
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This experiment was conducted to study the relationship between the energy value and the nitrogen content in the rat urine. Thirteen rats for ad libitum feeding group and 16 rats for two-meal group were employed in this experiment. The experimental period consisted of 22 days with a preliminary period of 10 days. During the last eight days of the experiment the nine was collected quantitatively.
The rats fed two-meal per day excreted significanrly (p$lt;0.005) more nitrogen and energy in the urine than those fed ad libitum.
A linear relationship between the energy concentration and nitrogen content was found. The urinary energy value was increased as the urinary nitrogen content was increased. A prediction equation was derived to compute the energy value from the content of nitrogen as follows:
Y = 8.924 X + 0.182 S_(y.x) = 0.788
where Y = urinary energy(㎉/100㎖)
X = urinary nitrogen(gm/100㎖)
Since the standard deviation of estimate is in smell magnitude (0.788 ㎉) when it is compared with the amount of intake of gross energy, digestible energy or metabolizable energy, this equation can be used safely to estimate the energy value from nitrogen content. Consequently, considerable around of time and labor for the actual determination of energy can be saved.
The ratio of energy to nitrogen was found to be 9.4 for ad adlibitum group and 8.6 for two-meal group. No significant difference between two group in this respect was observed.
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